Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism / 中国应用生理学杂志

OBJECTIVE@#To investigate the effects of aminooxyacetic acid (AOAA) on learning and memory ability and possible mechanisms in rats with chronic alcoholism.@*METHODS@#Sixty SD male rats were randomly divided into three groups on average.The model group rats and the remedy group rats were fed with the water containing (v/v) 6% alcohol for 28 days.After 14 days, the remedy group rats were treated with AOAA (5 mg/kg·d) by intraperitoneal injection once a day for 14 days and the other two group rats were treated with the equal amount of saline by intraperitoneal injection every day.Five days before the end of the experiment, the water maze test was carried out to test the learning and memory ability of rats for 5 days.Subsequently, the content of HS, the activity of ATP enzyme and the expression of 5-HT in hippocampus were measured.@*RESULTS@#Compared with the rats in the control group, the latency and the swimming distance of the 2nd to the 4th day, the content of HS in hippocampus of rats in the model group were all increased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were decreased (<0.01).Compared with the rats in the model group, the latency and the swimming distance of the 2nd to the 4th day, the content of HS in hippocampus of the rats in the remedy group were decreased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were increased (<0.01).@*CONCLUSIONS@#AOAA could alleviate the symptoms of chronic alcoholism rats, which may be related to the effects of AOAA on the content of HS, the mitochondrial enzyme activity and the expression of 5-HT in hippocampus.

Effect of Beclin-1 shRNA on hypoxia-induced autophagy in SH-SY5Y cells / 中国应用生理学杂志

OBJECTIVE@#To observe the effect of hypoxia on autophagy in Beclin-1-knockdown SH-SY5Y cells by constructing a stable transfected SH-SY5Y cell lines of silencing Beclin-1 gene.@*METHODS@#Beclin-1shRNA lentiviral vector and negative control lentiviral vector were constructed; the vector was transfected into SH-SY5Y cells; then the expression of Beclin-1 mRNA was detected by RT-PCR, the level of Beclin-1 protein was detected by Western blot. CCK-8 method was used to determine the effect of Beclin-1 knockdown on the viability of SH-SY5Y cells. Next, the blank control, negative control and transfected cells were cultured under 21% normoxia and 5% hypoxia conditions. The expression of LC3 protein in each group was detected by Western blot and the autophagic bodies were observed by electron microscopy.@*RESULTS@#Beclin-1 shRNA significantly inhibited the expression of Beclin-1 mRNA and protein in SH-SY5Y cells; after silencing Beclin 1 gene, the survival rate of Beclin-1 shRNA group cells was no different from that of negative control (NC) group. After 5% hypoxia treatment, compared with NC group, the ratio of LC3Ⅱ/LC3Ⅰand the number of autophagy bodies were all decreased in Beclin-1 shRNA group.@*CONCLUSIONS@#Beclin-1 knockdown SH-SY5Y cell lines and negative control cell lines were successfully established. Lentivirus-mediated Beclin-1 shRNA has no effect on the viability of SH-SY5Y cells, but can inhibit hypoxia-induced autophagy.

Analysis of publication of West China Journal of Stomatology from 2000 to 2005 / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the characteristic of publication of West China Journal of Stomatology (WCJS).</p><p><b>METHODS</b>Making use of literature metrology, a comprehensive analysis of the information publication of West China Journal of Stomatology from 2000 to 2005 was made.</p><p><b>RESULTS</b>The quantity of articles published from 2000 to 2005 in West China Journal of Stomatology was 964, the average fund paper ratio was 0.419, 96.78% of articles had more than one author, and the average paper author cooperating degree was 4.01. The authors scattered in 31 provinces.</p><p><b>CONCLUSION</b>West China Journal of Stomatology is one of the most important information source of stomatology.</p>